Sumerianz Journal of Biotechnology

Online ISSN: 2617-3050
Print ISSN: 2617-3123

Quarterly Published (4 Issues Per Year)

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Volume 2 Issue 3 (2019)

Occurrence and Incidence of Major Potato Viruses in Bangladesh and their Molecular Detection

Authors : Md. Belal Hossain ; Ratna Akter ; Kaniz Fatema ; Ibne-Siam-Joy ; Mamunur Rashid
Incidence of major potato viruses in eight major potato growing areas of Bangladesh, viz.  Panchagarh, Thakurgaon, Nilphamari, Rangpur, Bogra, Chapainawabgonj, Pabna and Munshiganj were investigated in field samples. Overall,  three  upazilla  (sub-districts)  from  each district  (which  were  at  least  20  km  apart)  were selected  for  samples  collection  and  disease incidence monitoring.  A  total  of  240  samples  were  collected  on  the basis  of  virus  and  viral  like  symptoms.  Collected samples were storage at 40C and analyzed in Molecular Biology and Plant Virology lab, Central Research Laboratory of Sher-e-Bangla Agricultural University (SAU), Dhaka. Enzyme  Linked  Immunosorbent  Assay  (ELISA)  tests  revealed  that  Potato  leaf  roll  virus  (PLRV)  was most predominant  virus  followed by Potato  virus  Y (PVY) and Potato virus X (PVX). During the year 2017-18, the  relative  frequency  of  infection  by  PLRV and PVY  was  31 and 2%  of  infected  samples respectively. Single, double and triple infections were 34, 45 and 2.0% respectively. Infection of detected potato viruses in all investigated districts with different percentage was almost similar. Major potato viruses’ symptoms that appear at investigated areas are PLRV, PVY and PVX and their relative incidences level in random samples were severe to moderate. In all investigated areas, PLRV and PVY appeared in severe to moderate level and their % incidence was (18 & 41%) and (3 & 17%) respectively, while the PVX was appeared in moderate level and % incidence of PVX 18%.  In this study, the sources of potato seed tubers was also studied and observed that in most of the cases farmers of selected areas are using continuously same field and used their own seed tubers that was kept in cold storage condition and % frequency was 67.6%. PLRV and PVY were also detected via reverse transcriptase polymerase chain reaction (RT-PCR). A 346 bp and 480 bp amplicon of PLRV and PVY- coat protein (CP) gene was amplified and the nucleotide sequences of amplified.

Pages: 16-24