Sumerianz Journal of Biotechnology

Online ISSN: 2617-3050
Print ISSN: 2617-3123

Quarterly Published (4 Issues Per Year)

Journal Website:


Volume 2 Issue 9 (2019)

Molecular Studies of Bacterial Isolates of Some Benthic Seafood Harvested from Oil Producing Areas in the Niger Delta, Nigeria

Authors : Daworiye S. P. ; Braide W. ; Nwachukwu I. N. ; Okorondu S. I.
The benthic region of the sea consists of a wide variety of organisms including the seafood (molluscs and crustaceans), which serve as a rich source of nutrients for man and other organisms. Oil exploration and exploitation activities in the Niger Delta region of Nigeria has resulted in environmental, socioeconomic and physical damage of the ecosystem over. Bacteria were isolated from selected benthic seafood harvested from oil producing areas in the Niger Delta using standard methods. The molecular identification of the isolates were carried out using the BigDye Terminator kit and the bioinformatics algorithm Trace Edit. Similar sequences from the National Center for Biotechnology Information (NCBI) database were matched using Basic Local Alignment Search Tool Nucleotide (BLASTN).  Sequences were aligned using MAFFT and the evolutionary history inferred using the Neighbour-Joining method. The extracted DNA samples all had acceptable range of DNA concentration and DNA purity. All the twelve organisms isolated and identified were associated with contaminated water and seafood. Identified bacteria include Pseudomonas aeruginosa, Myroides odorantimimus, Bacillus flexus, Bacillus velezensis, Shewanella chilikensis, Enterobacter hormaechei, Enterobacter cloacae, Escherichia fergusonii, Escherichia coli and Klebsiella pneumoniae. Some of the isolates had been reported to be pathogenic and food spoilage. Environmentally friendly practices in oil production will ensure a healthy aquatic environment in the Niger Delta.

Pages: 78-81

Environmental Monitoring of Atmospheric Air Pollution with Radionuclides Using Moss Bioindicators

Authors : Sh. A. Topchiyeva ; M. A. Mehrabova ; Karimova R. Z. ; Y?ld?z L. Z.
The purpose of our research was to identify the degree of air pollution with radionuclides using moss bioindicators. We studied samples of moss and soil (near Mingechaur, Baku) to identify the bio-indicator properties of moss and for bioindication of air and soil. We studied samples of moss, soil and water near Mingechaur city and Baku city regions. The activity of radionuclides were determined on Gamma spectrometer. Determination of content and activity of the radionuclides in the tests of soil, plant and water, was carried out at "Canberra" (USA)γ-spectrometer with high a purity germanium detector (HP Ge). It was determined the activity of radionuclides (7Be, 40K, 60Co, 208Tl, 134Cs, 137Cs, 210Bi, 212Bi, 212Pb) in the samples of of moss, soil and water samples taken from the Mingechaur region  and  in Baku region of the Azerbaijan Republic. As a result of the research it was revealedе the activity of 40K was more than other isotopes (648,1 Bq/kg). It was found out 7Be of activity 79.08 Bq/kg, 214Pb of activity 62.62Bq/kg, 214Bi of activity 28.74 Bq/kg, 137Cs of activity 14.01 Bq/kg, 134Cs of activity 12.15 Bq/kg, 60Co of activity 11.34 Bq/kg and oters, which were more in Mingechaur region than in Baku.

Pages: 75-77